IGV Genome Browser App

Author

Jenny L. Smith

1 IGV

Use VSCode to remote connect to the PromethION instrument by remote-ssh in vscode. Then launch a locally hosted IGV webserver.

Please ask Marika Guercio or Francesca Benini for the remote connection details.

Important
  1. Set-up your environment by running these commands:

export PROJ_DIR=$HOME/Documents/quintarelli_c && source $PROJ_DIR/.bash_profile

2 To launch the application

  1. On the commandline enter the following:
IGV_WEBAPP=$PROJ_DIR/opt/igv-webapp.3.0.7
npx http-server -t30 -c-1 -a localhost $IGV_WEBAPP
  1. Select the PORTS tab from the IGV terminal pane.

  2. Right click (cmd+click on MacOS) on the blue hyperlink localhost:XXXX

  3. Select Open in Browser from the pull-down menu

  4. VCF files and processed WGS data is included in a track called WGS Nanopore T-ALL in the pull down-menu

3 IGV Documentation and Resources

  1. Short video tutorials

  2. IGV Application User Guide

  • The web application uses the same user guide as the desktop application
  1. IGV Web application documentation
  • Mainly for deployment docs